1.2D肠道干细胞和3D气液界面类器官培养体系

图1：肠道干细胞气液界面类器官技术体系示意图（Wang et al., Nature, 2015）

2.二维肿瘤细胞系和三维肿瘤类器官技术平台

图2：二维肿瘤细胞系和三维肿瘤类器官技术平台示意图（Zhao et al., Advanced Science, 2022）

3.精准医疗的新靶向——原发肿瘤中的转移种子

图3. 系统探究结直肠癌原发肿瘤中的转移种子 (Zhao et al., JEM, 2024）

1. Yuanyuan Zhao^#, Bing Zhang^#, Yiming Ma^#, Mengmeng Guo, Fuqiang Zhao, Jianan Chen, Bingzhi Wang, Hua Jin, Fulai Zhou, Jiawei Guan, Qian Zhao, Qian Liu*, Hongying Wang*, Fangqing Zhao*, Xia Wang*. Distinct molecular profiles drive multifaceted characteristics of colorectal cancer metastatic seeds. Journal of Experimental Medicine (2024 May 6;221(5):e20231359. doi: 10.1084/jem.20231359.)

2. Chuandong Liu^#, Jie Li^#, Hua Jin^#, Qian Zhao^#, Fangle Li, Zurui Huang, Boyuan Mei, Wenxuan Gong*, Xia Wang*, Dali Han*. Colonic stem cell from severe ulcerative colitis maintains environment-independent immune activation by altering chromatin accessibility and global m 6A loss. Life Medicine, 2023. 2 (4):1-14.

3. Yuanyuan Zhao^#, Mengmeng Guo^#, Fuqiang Zhao, Qian Liu and Xia Wang*. Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer. Cell Communication and Signaling, 2023, 21:186.

4. Aging Biomarker Consortium, Hainan Bao^#, Jiani Cao^#, Mengting Chen^#, Min Chen^#, Wei Chen^#, Xiao Chen^#, Yanhao Chen^#, Yu Chen^#, Yutian Chen^#, Zhiyang Chen^#, Jagadish K. Chhetri^#, Yingjie Ding^#, Junlin Feng^#, Jun Guo^#, Mengmeng Guo^#, Chuting He^#, Yujuan Jia^#, Haiping Jiang^#, Ying Jing^#, Dingfeng Li^#, Jiaming Li^#, Jingyi Li^#, Qinhao Liang^#, Rui Liang^#, Feng Liu^#, Xiaoqian Liu^#, Zuojun Liu^#, Oscar Junhong Luo^#, Jianwei Lv^#, Jingyi Ma^#, Kehang Mao^#, Jiawei Nie^#, Xinhua Qiao^#, Xinpei Sun^#, Xiaoqiang Tang^#, Jianfang Wang^#, Qiaoran Wang^#, Siyuan Wang^#, Xuan Wang^#, Yaning Wang^#, Yuhan Wang^#, Rimo Wu^#, Kai Xia^#, Fu-Hui Xiao^#, Lingyan Xu^#, Yingying Xu^#, Haoteng Yan^#, Liang Yang^#, Ruici Yang^#, Yuanxin Yang^#, Yilin Ying^#, Le Zhang^#, Weiwei Zhang^#, Wenwan Zhang^#, Xing Zhang^#, Zhuo Zhang^#, Min Zhou^#, Rui Zhou^#, Qingchen Zhu^#, Zhengmao Zhu^#, Feng Cao*, Zhongwei Cao*, Piu Chan*, Chang Chen*, Guobing Chen*, Hou-Zao Chen*, Jun Chen*, Weimin Ci*, Bi-Sen Ding*, Qiurong Ding*, Feng Gao*, Jing-Dong J. Han*, Kai Huang*, Zhenyu Ju*, Qing-Peng Kong*, Ji Li*, Jian Li*, Xin Li*, Baohua Liu*, Feng Liu*, Lin Liu*, Qiang Liu*, Qiang Liu*, Xingguo Liu*, Yong Liu*, Xianghang Luo*, Shuai Ma*, Xinran Ma*, Zhiyong Mao*, Jing Nie*, Yaojin Peng*, Jing Qu*, Jie Ren*, Ruibao Ren*, Moshi Song*, Zhou Songyang*, Yi Eve Sun*, Yu Sun*, Mei Tian*, Shusen Wang*, Si Wang*, Xia Wang*, Xiaoning Wang*, Yan-Jiang Wang*, Yunfang Wang*, Catherine C. L. Wong*, Andy Peng Xiang*, Yichuan Xiao*, Zhengwei Xie*, Daichao Xu*, Jing Ye*, Rui Yue*, Cuntai Zhang*, Hongbo Zhang*, Liang Zhang*, Weiqi Zhang*, Yong Zhang*, Yun-Wu Zhang*, Zhuohua Zhang*, Tongbiao Zhao*, Yuzheng Zhao*, Dahai Zhu*, Weiguo Zou*, Gang Pei* & Guang-Hui Liu*. Biomarkers of aging. Science China Life Sciences. 2023. 66: 893-1066.

5. Yuanyuan Zhao^#, Bing Zhang^#, Yiming Ma, Fuqiang Zhao, Jianan Chen, Bingzhi Wang, Hua Jin, Fulai Zhou, Jiawei Guan, Qian Zhao, Hongying Wang, Qian Liu*, Fangqing Zhao*, Xia Wang*. Colorectal cancer patient-derived 2D and 3D models efficiently recapitulate inter- and intra-tumoral heterogeneity. Advanced Science. 2022 (DOI: 10.1002/advs.202201539)

6. Qian Zhao^#, Jiawei Guan, Xia Wang*. Intestinal stem cells and intestinal organoids. Journal of Genetics and Genomics. 2020. 47(6):289-299. (Review)

7. Yamamoto Y*, Wang X*(*Co-first authors), Bertrand D, Kern F, Zhang T, Deluba M, Srivastava S, Khor CC, Hu YY, Wilson L, Blaszyk H, Rolshud D, Ming T,Liu JJ, Howitt B, Vincent M, Crum CP, Nagarajan N, Ho KY, McKeon F, and Xian W. Mutational spectrum of Barrett’s stem cells suggests paths to initiation of a precancerous lesion. Nature Communications. 2016. doi: 10.1038/ncomms10380.

8. Wang X*, Yamamoto Y*(*Co-first authors), Wilson LH, Zhang T, Howitt B, Ning G, Hong Y, Khor CC, Chevalier B, Bertrand D, Nagarajan N, Sylvester FA, Farrow MA, Lacy DB, Ho KY, Crum CP, McKeon F and Xian W. Cloning and variation of ground state intestinal stem cells. Nature. 2015. 522(7555):173-178.

9. Wang X*, Ouyang H*, Yamamoto Y*, (*Co-first authors), Kumar PA, Wei TS, Dagher R, Vincent M, Lu X, Bellizzi AM, Ho KY, Crum CP, Xian W, McKeon F. Residual embryonic cells as precursors of a Barrett's-like metaplasia. Cell. 2011. 145(7):1023-35. Featured Article in Cell. Highlighted in Nature Reviews Cancer. Faculty 1000 Biology: 10 (Exceptional).

10. Wang, X and Dai, J. Isoforms of OCT4 contribute to the confusing diversity in stem cell biology. Stem Cells. 2010. 28(5):885-93. (Invited Review)

11. Gao, Y., Wang, X. *(* Co-first authors), Han, J., Xiao, Z., Chen, B., Su, G., Dai, J. The novel OCT4 spliced variant OCT4B1 can generate three protein isoforms by alternative splicing into OCT4B. J Genet Genomics. 2010. 37(7):461-5

12. Zhang, J., Wang, X. *(* Co-first authors), Chen, B., Xiao, Z., Li, W., Lu, Y. and Dai, J. The human pluripotency gene NANOG/NANOGP8 is expressed in gastric cancer and associated with tumor development. Oncology Letters. 2010. 1(3):457-463.

13. Wang, X., Zhao, Y., Xiao, Z., Chen, B., Wei, Z., Wang, B., Zhang, J., Han, J., Gao, Y., Li, L., Zhao, H., Zhao, W., Lin, H. and Dai, J. Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response. Stem Cells. 2009. 27(6):1265-75.

14. Zhang, J.*, Wang, X.* (* Co-first authors), Han, J., Chen, B., Wang, B., Suo, G. and Dai J. NANOGP8 is a retrogene expressed in cancers. FEBS Journal. 2006. 273(8): 1723-30.